Leave a cup of water in the microwave with the plates.
The steam will kill the spores.
Vapor will condense on all of the surfaces, since everything in the microwave
will be colder than the water vapor because the water is the only thing
absorbing microwaves.
The vapor constantly condensing and re-boiling off of the surfaces will keep
all of the surfaces pretty close to the boiling point for the entire three
minutes. It will also cause more damage to the spores and cells than simple
heat alone, much like cavitation can cause damage with ultrasound.
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Get a free science project every week! "http://scitoys.com/newsletter.html"On Thu, Apr 26, 2012 at 5:22 PM, John Griessen <john@industromatic.com> wrote:
On 04/26/2012 04:49 PM, Cathal Garvey wrote:Maybe a good recipe/protocol for cooking spores with efficient microwave energy
Spores often have a low our negligible water content, and "wet heat" in the form of superheated steam is a great way to kill them.
resonating on water molecules is to coat them with some kind of wet gel like agar/detergent first?
But then you'd have agar/detergent coated sterile micro plates.
what is more water absorbing and innocuous than that?
Is there anything that would make spores absorb water, and not coat the plastic surface where it was
pure and clean? Or something that leaves a layer so thin it will not make much heat to distort the
plastic under microwave energy, but it will give the spores some water to heat up with?
the alcohol treatment is sounding better than microwaves so far.
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