Alcohol doesn't really affect spores much. Wet heat with steam followed by alcohol spray is a nice setup: rinsing with alcohol assists in rapid drying and helps remove water-insoluble residues. I doubt you'd get much better without a more complicated or expensive setup.
John Griessen <john@industromatic.com> wrote:
>On 04/26/2012 04:49 PM, Cathal Garvey wrote:
>> Spores often have a low our negligible water content, and "wet heat"
>in the form of superheated steam is a great way to kill them.
>
>Maybe a good recipe/protocol for cooking spores with efficient
>microwave energy
>resonating on water molecules is to coat them with some kind of wet gel
>like agar/detergent first?
>
>But then you'd have agar/detergent coated sterile micro plates.
>
>what is more water absorbing and innocuous than that?
>
>Is there anything that would make spores absorb water, and not coat the
>plastic surface where it was
>pure and clean? Or something that leaves a layer so thin it will not
>make much heat to distort the
>plastic under microwave energy, but it will give the spores some water
>to heat up with?
>
>the alcohol treatment is sounding better than microwaves so far.
>
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Re: [DIYbio] Recycling micro plates
3:01 AM |
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