[DIYbio] Re: Help required in sourcing cheap cell culture reagents

Well, to be fair, he made little progress in extraction.  Howard Florey and Ernst Chain were the folks who eventually established an industrial extraction process.
Veterinary grade stock is probably your best bet.

On Thursday, 3 May 2012 15:29:09 UTC+1, Mega wrote:

Well, Alexander Flemming could do it many years ago ;)

Am Donnerstag, 3. Mai 2012 02:40:49 UTC+2 schrieb Mary:

Hi All

I plan do do some basis cell culture but doent want to spend a fortune on the basis reagents needed to do this work and plan to source my ingredients from Health Food shops. I am looking for cheap and effective substitutes for the following:

1) L-Glutamine solution (can I make my own using health food glutamine?)

2) Penicillin/streptomycin (are there any natural alternatives?)

3) HEPES buffer

4) Hanks salt solution

5) Trypsin-EDTA solution used to subculture cells

6) Cell culture medium (can I make my own using health food supplies?)

All help is appreciated!

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