[DIYbio] Re: Transformation questions

Hey.

Did another transformation yesterday @university, and they are glowing. not very bright, but well visible.  Amazing!!!
I got ready lb-amp plates from a PhD I know.
I was also given competent -80°C colis instead of cacl2 trafo.

All the best,




Am Montag, 23. Januar 2012 21:02:53 UTC+1 schrieb Mega:

Hello @all,

When I want to transform bacteria they have to be in exponential
growth.

So usually you'd have do do an over-night culture @ 37°C.


I don't have a breeding box, will they be in exponential growth also
at 21°C (but some 20 hours instead of just 12)???


Best regards,

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/0zCpTq4AyR0J.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment