Re: [DIYbio] freezing and storing bacteria

Nicely done!

The standard LONG term storage is storage in 50% glycerol in water and placed at -80ºC. -20ºC may be fine (standard household freezer temperature) but household freezers fluctuate in temperature to go through a defrost cycle which is not good for your bacterial stocks.

In the fridge the bacteria will continue to grow but very slowly -- the upshot is that they will lose your plasmid within a month or two. Don't store them in the fridge.

good luck!

jordan





On May 9, 2012, at 12:36 PM, Mega wrote:

> As I finally have my glowing bacteria, I would like to store some of them in the freezer in case that the Plasmid is thrown away by the bacteria.
>
> How do you store your bacteria?
>
>
> Take a toothpick of bacteria, put it into glycerol and then into the fridge??
>
> Or just agar in an eppi, and the bacteria on agar. In the fridge-> ready?
>
>
> Thanks,
>
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