I put my bacterial stocks into a foam shipping container with a coldpack in it, and put that into the freezer. Grow bacterial culture to mid-log phase (just so that the greatest number of cells are viable and actively dividing), then dilute 50/50 with glycerol, and toss it in the freezer. I usually try to renew stores every year, just to make sure they're OK, but I've restored stocks as old as 3 years without any issue.
--Derek
On Wednesday, 9 May 2012 09:50:37 UTC-7, Jordan Miller wrote:
Nicely done!--
The standard LONG term storage is storage in 50% glycerol in water and placed at -80ºC. -20ºC may be fine (standard household freezer temperature) but household freezers fluctuate in temperature to go through a defrost cycle which is not good for your bacterial stocks.
In the fridge the bacteria will continue to grow but very slowly -- the upshot is that they will lose your plasmid within a month or two. Don't store them in the fridge.
good luck!
jordan
On May 9, 2012, at 12:36 PM, Mega wrote:
> As I finally have my glowing bacteria, I would like to store some of them in the freezer in case that the Plasmid is thrown away by the bacteria.
>
> How do you store your bacteria?
>
>
> Take a toothpick of bacteria, put it into glycerol and then into the fridge??
>
> Or just agar in an eppi, and the bacteria on agar. In the fridge-> ready?
>
>
> Thanks,
>
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