Re: [DIYbio] freezing and storing bacteria

On Wed, May 9, 2012 at 3:03 PM, Andreas Sturm <masterstorm123@gmail.com> wrote:
> Yeah, that too... (strain & plasmid)
>

For the bacterial cell culture storage, I add approximately 300uL
glycerol to 1.5mL tube and add 500uL from overnight inoculation
(usually from our 100mL maxiprep culture). I vortex for complete
mixing and immediately rush to the -80C and freeze it down. I hear
glycerol will destroy the cells unless frozen down.

Plasmid: you have to extract the DNA somehow and elute in TE buffer.
Store in -20C freezer.

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