I'll google how to do that and maybe I'll do a huge miniprep to get some (or a lot of ;) ) plasmids.
I don't know why the bacteria in the middle of the dish don't glow strongly so that you can see it. Maybe they have consumed all of the sugars of the agar?
That would be an idea: whenever I need glowing bacteria, I do a transformation... Well, if I can grow me enough plasmids, why not?
But therefore I'll need EDTA, SLS, etc. ...
Once or twice I surely can try at university, but someday I'll have to get my own chemicals :)
2012/5/9 Avery louie <inactive.e@gmail.com>
Also, send us some pictures! I would love to see your work.
--AOn Wed, May 9, 2012 at 1:55 PM, Avery louie <inactive.e@gmail.com> wrote:
It seems like you want to maintain the plasmid, not the strain! Consider doing a plasmid extraction, and freezing/drying the plasmid.
--AOn Wed, May 9, 2012 at 1:22 PM, Derek <derekja@gmail.com> wrote:
I put my bacterial stocks into a foam shipping container with a coldpack in it, and put that into the freezer. Grow bacterial culture to mid-log phase (just so that the greatest number of cells are viable and actively dividing), then dilute 50/50 with glycerol, and toss it in the freezer. I usually try to renew stores every year, just to make sure they're OK, but I've restored stocks as old as 3 years without any issue.
--DerekTo view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/hfmw4k6ZIdcJ.
On Wednesday, 9 May 2012 09:50:37 UTC-7, Jordan Miller wrote:Nicely done!--
The standard LONG term storage is storage in 50% glycerol in water and placed at -80ºC. -20ºC may be fine (standard household freezer temperature) but household freezers fluctuate in temperature to go through a defrost cycle which is not good for your bacterial stocks.
In the fridge the bacteria will continue to grow but very slowly -- the upshot is that they will lose your plasmid within a month or two. Don't store them in the fridge.
good luck!
jordan
On May 9, 2012, at 12:36 PM, Mega wrote:
> As I finally have my glowing bacteria, I would like to store some of them in the freezer in case that the Plasmid is thrown away by the bacteria.
>
> How do you store your bacteria?
>
>
> Take a toothpick of bacteria, put it into glycerol and then into the fridge??
>
> Or just agar in an eppi, and the bacteria on agar. In the fridge-> ready?
>
>
> Thanks,
>
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