Re: [DIYbio] How to get surface proteins from one bacteria to another? Can somebody help?

While that woulda make an interesting project, I don't feel it's appropriate for 'DIYbio', as we generally avoid projects dealing with potential or known human pathogens. Not only is it illegal in most places to do this without a license, it's genuinely a bad idea to do it without professional training.

Not only that, it's be hard! You won't be able to get those genes synthesised because they're clearly derived from a pathogen. When the synthesis company run a BLAST on it and see 'salmonella', you're unlikely to convince them to make and ship it to you. That means you'd be stuck working with the pathogen itself, not a very friendly situation to be in.

I know someone on this list is looking at making vaccines to a common lizard-killing pathogen- fascinating project idea as no known cases of human infection have occurred. Provided one was careful, that would seem a good starting point for a more 'DIYbio' project.

Nils Reither <nilsreither@gmail.com> wrote:

>Hi
>I wonder if it is possible to get the genes from the O and H antigen
>proteins in Salmonalla bacteria transferred to more harmless bacteria
>such
>as Bacillus subtilus or Lactobacillus.
>This way a risk free immunisation would be possible.
>
>Cheers
>Nils Reither, veterinarian
>
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