Re: [DIYbio] Re: Transformation questions

Hey Andreas,
Very delayed I know, but I just wanted to congratulate you on a job well done! Your enthusiasm and dedication is awesome. :)

Mega <masterstorm123@gmail.com> wrote:

>Hey.
>
>Did another transformation yesterday @university, and they are glowing.
>not
>very bright, but well visible. Amazing!!!
>I got ready lb-amp plates from a PhD I know.
>I was also given competent -80°C colis instead of cacl2 trafo.
>
>All the best,
>
>
>
>
>Am Montag, 23. Januar 2012 21:02:53 UTC+1 schrieb Mega:
>>
>> Hello @all,
>>
>> When I want to transform bacteria they have to be in exponential
>> growth.
>>
>> So usually you'd have do do an over-night culture @ 37°C.
>>
>>
>> I don't have a breeding box, will they be in exponential growth also
>> at 21°C (but some 20 hours instead of just 12)???
>>
>>
>> Best regards,
>
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