It's me again :D
I was wondering what the problems were when introducing a bacterial gene into an eukaryont (easiest case: GFP or lux into yeast).
I identified some:
1) Codon usage:
When the codon bias is very different between them, it will give lower expression levels.
But it's not a show-stopper as the protein is still produced.
2) Ribosome binding Site:
According to this, it's not a big problem for bacteria->eukarotes.
http://www.invitrogen.com/site/us/en/home/References/Ambion-Tech-Support/translation-systems/general-articles/ribosomal-binding-site-sequence-requirements.html
"Our data demonstrate that in contrast to the E. coli ribosome, which preferentially recognizes the Shine-Dalgarno sequence, eukaryotic ribosomes (such as those found in retic lysate) can efficiently use either the Shine-Dalgarno or the Kozak ribosomal binding sites."
in the direction plant gene/mushroom gene/human gene -> bacteria this will be a problem.
Is my thinking about RBS correct??
3) Promoter:
A bad promoter will give you bad results.
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