That's an awsome idea....
Because as a DIYer it's very difficult to get X-Gal. I either as my proffessor (again and again and again, he'll hate me :D ) or buy it on the internet. Some companies won't ship it to residental adresses, and if they do, delivery costgs are enormous. And also the X-Gal won't be cheap.... A pigment, on the other hand is synthesied out of nothing than LB-Medium. That would be a cheap, most-easy-to handle way of reporter gene!!
I think it is possible to design primers for PCR to cut the gene into two genes. Handle if as the gene were two genes. So it will be cut in the middle, won't it. There the primer should have the sequence of a restriction site.
But maybe there's another problem: If the vector plasmid has MCS, there may be 2 of each restr-site?? (If you take the RE in the middle).
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