Hello Everyone,
In the past, there has been interest in a more advanced "synthetic" or molecular bio class involving not only adding foreign dna to new chassis, but actually manipulating the DNA, as far as restriction digests, ligation, and PCR. I am working on developing this course, but just like the last class that was developed, I want to invite a few people to join me on this project.
What:
We will start by growing different bacteria that have genes that we want. Then we will clone the genes out using PCR, and eventually digest and ligate them into a plasmid. We will take that plasmid and put it in e. Coli. BAM! We will have made a transgenic organism with our own cloned DNA. I will be cloning a pigment gene from a Rhodococcus, and you are welcome to do that as well, but it would be fun to do a few genes- maybe a lacZ' or an ecoR1. I have done this before, but not at bosslab.
Who:
Five people with a little bit of molecular biology background (biotech 101, read a book, experience reccomended but not required), who have $150 to spend on this project, and lots of time weekday nights. Money goes to reagents for PCR, primers, ligase, pcr cleanup, and twoards a scale/uv vis spec for dna quantification.
When:
When you can come in, but realistically we can break tasks up so that people can skip days and have their lab mates cover them. Once the parts are in, you can even complete the project on your own time, if you feel comfortable. The project will start when enough people to do it have been collected.
If you are interested, just email me or reply all to the list. Project will begin when there are enough people.
--Avery
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