Thanks all for advice/links. As I continue to work on this I will evaluate feasibility of kits, but I will definitely document. I know IOrodeo has kits but I think $300 is a little out of budget. Thanks to IOrodeo though for making there schematics completely available.
Anyone have the Gelbox 2.0 laser cut schematics - I found the sketch up 3d files, but no laser files.
Ravi
On Tuesday, June 5, 2012 10:18:52 AM UTC-4, Pieter wrote:
The full instructions can be found on instructables:
- http://www.instructables.com/id/Gel-electrophoresis-system- mini/?ALLSTEPS
- http://www.instructables.com/id/UV-Transilluminator/? ALLSTEPS
Following these steps would probably also lead you to a fine gelbox: http://learn.genetics.utah.edu/content/labs/gel/build_ gel_box.pdf
The instructables are pretty nice, but it is sometimes hard to get all the components. Perhaps we should make a kit, as a supplement to the OpenPCR
On Tuesday, 5 June 2012 10:43:01 UTC+2, cory....@gmail.com wrote:I don't know anything about the Open Gelbox 2.0 but IORodeo has a gel
box which they give away the design files for (
http://www.iorodeo.com/content/mini-gel- )electrophoresis-system
including the spec sheet, Open SCAD files and laser-cutting files.
They also have python scripts in one of their Bitbucket repositories (
https://bitbucket.org/iorodeo/transilluminator_enclosure/src ) which
allow you to input different dimensions and it will generate the SCAD
files automatically. So if you want a larger or smaller box than the
default, you can easily make that happen.
As for the blue and amber filters for an LED illuminator, I had good
luck using gel filters (the colored things you see covering lights at
theater performances or movie sets). I got them from this guy on ebay
http://www.ebay.com/itm/390061292911?ssPageName=STRK: MEWAX:IT&_trksid=p3984.m1423. l2649
They have a huge selection of colors and they are very cheap ($6 for
4sq ft). If you want I can go through my notebook and find which
specific colors worked best.
No need to use an arduino to control the LEDs. If you have a DC power
supply, hook a few LEDs in series with a resistor and add a switch if
you want. Much cheaper.
Also, if you're interested in making a UV illuminator, IORodeo has
design files for that as well
http://www.iorodeo.com/content/transilluminator- enclosure
-cory
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