Re: [DIYbio] Re: DIYbio projects

I'm working on this. I'd share the idea right away, but I want to
implement it first to guarantee that I've established "prior art" beyond
reasonable doubt.

The problem is, there are great methods out there, but they're patented
to oblivion. Consider Maltose-Binding Protein: You could, in principal,
just use cold (insoluble) potato starch to purify proteins. But, it's
patented in the EU for another few years at least, and possibly in the
US for the time being, too.

I've got something up my sleeve, but I don't want to put it into the
public domain until I'm sure nobody can steal/patent it and prevent it
from being used by the community. I'm sure patent apologists will insist
that this is unreasonable, but history says it's not.

On 04/06/12 21:12, Cory Tobin wrote:
>> My suggestions would be:
>> - Enzymes commonly used in the lab that would save a lot of money when
>> produced by yourself, such as DNA restriction and polymerase, some are
>> available
>
> I like the idea of people being able to produce their own enzymes. It
> would be great if we could produce our own Taq, ligase and the four
> BioBrick restriction enzymes (EcoRI, XbaI, SpeI, PstI). With those 6
> enzymes one could not only work with most BioBrick parts but also
> bootstrap their lab and produce any additional enzymes they need.
>
> My main concern with this right now is the lack of inexpensive methods
> for purifying the protein. Most column based methods are expensive
> (NTA, etc). But I don't have much experience purifying enzymes aside
> from using kits. Maybe someone with more experience knows of an
> inexpensive method for producing enzymes pure enough to be used for
> BioBricks? This project http://2009.igem.org/Team:Washington/Project
> looked really promising but it looks like they never really got the
> system working and quit working on it.
>
>
> -cory
>

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