Re: [DIYbio] Re: DIYbio projects

Thanks for the great suggestions, I'll start putting a list together this week.

231.313.9062 // @100ideas // sent from my rotary phone

On Jun 3, 2012, at 3:40 AM, Pieter <pietervanboheemen@gmail.com> wrote:

There is a groupparts tag:  http://partsregistry.org/Help:Wiki_Tag_groupparts 

During our iGEM participation we tried to build an iphone app for making browsing the registry more user friendly, unfortunately the API responsiveness was so slow that it was too frustrating. It might have been improved over the past 2 years. Anyone who wants to retry can start out here:  http://partsregistry.org/Registry_API 

When selecting the top 48 biobricks I would start out with the finalist teams. In general they contributed well documented parts. Also, quickly scan the biobrick RFCs for usefull parts http://biobricks.org/programs/technical-standards-framework/

My suggestions would be:

- Enzymes commonly used in the lab that would save a lot of money when produced by yourself, such as DNA restriction and polymerase, some are available on http://partsregistry.org/Protein_coding_sequences/DNA_modification

- Constitutive promotors of the Anderson family:  http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 

- High copy number plasmid:  http://partsregistry.org/wiki/index.php/Part:pSB1C3 

- Linker domains for isolating proteins: FLAG  http://partsregistry.org/wiki/index.php?title=Part:BBa_K128000, HA  http://partsregistry.org/wiki/index.php?title=Part:BBa_K128001 and HIS  http://partsregistry.org/wiki/index.php?title=Part:BBa_K128005 

On Sunday, 3 June 2012 10:00:09 UTC+2, cory....@gmail.com wrote:

> Is anyone interested in spending an afternoon cherry-picking the "best" 48
> parts with me?

Count me in.  I don't have much experience working with the parts - I
helped advise a team one year but that's it.  So I can't really
comment on the reliability of the parts from first-hand experience but
I might be able to help pick parts that _would_ be great, assuming
they work.

Maybe a first step is to assemble a spreadsheet of parts that have
been used successfully in fully-functional projects.  There's so much
stuff in the registry with no reviews and the search interface is
really really clunky to say the least.  I think it would be good to
have a reduced list of stuff that has been confirmed to work as
described by at least one iGEM team, preferably multiple teams.

Does anyone know of a place that lists the parts used by each iGEM
team?  That would save a lot of time digging through the individual
team wikis.


-cory

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/yt3rQxDxDjcJ.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.