Re: [DIYbio] Re: DIYbio projects

> My suggestions would be:
> - Enzymes commonly used in the lab that would save a lot of money when
> produced by yourself, such as DNA restriction and polymerase, some are
> available

I like the idea of people being able to produce their own enzymes. It
would be great if we could produce our own Taq, ligase and the four
BioBrick restriction enzymes (EcoRI, XbaI, SpeI, PstI). With those 6
enzymes one could not only work with most BioBrick parts but also
bootstrap their lab and produce any additional enzymes they need.

My main concern with this right now is the lack of inexpensive methods
for purifying the protein. Most column based methods are expensive
(NTA, etc). But I don't have much experience purifying enzymes aside
from using kits. Maybe someone with more experience knows of an
inexpensive method for producing enzymes pure enough to be used for
BioBricks? This project http://2009.igem.org/Team:Washington/Project
looked really promising but it looks like they never really got the
system working and quit working on it.


-cory

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