Re: [DIYbio] Re: How is Agrobacterium Tumefaciens grown? Is it possible to find in soil?

i also wanted to mention that the alternative transformation techniques use other patents outside of the Agrobacterium patent tree, though that's another long discussion.

On Thursday, June 28, 2012 5:59:01 AM UTC-4, Giovanni Lostumbo wrote:

Agrobacterium isn't the only plasmid-transferring microbe, there are ones that aren't gall forming, such as  Sinorhizobium meliloti (http://www.patentlens.net/daisy/bioforge_transbacter/3790/version/default/part/AttachmentData/data/Gene%20Transfer%20to%20Plants.taf.pdf) and Mesorhizobium loti:  http://www.patentlens.net/daisy/bioforge_transbacter/3586.html

On Monday, June 18, 2012 8:17:58 AM UTC-4, Cathal wrote:

It's not only the fact that lab strains don't cause disease; it's ease
of DNA manipulation.

Wild Agrobacterium plasmid is a complex mess of regulatory and transfer
sequences, and disease-causing DNA. The lab strains don't just have less
disease DNA, they also separate the transferring DNA from the "payload"
DNA, so there are actually two plasmids in the cell; one for the desired
plant DNA, another for sending the first via an infective pilus.

Definitely don't waste time trying to culture and re-hack a wild crown
gall bug; get your hands on disarmed and re-engineered lab strains and
you'll save a lot of time/money.

On 18/06/12 13:07, Matt DiLeo wrote:
> It's a naturally occurring pathogen, causing crown gall among other
> diseases, and there's plenty of information out in the plant pathology
> literature on where to find, identify and cultivate it - but you'll want a
> disarmed lab strain that doesn't causes disease.
>

--
www.indiebiotech.com
twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/hvRqoQAHzdcJ.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.