I agree for restriction enzymes. It would be great to have them just be sure to have corresponding modification partner. Without correct methylase many restriction nuclease will cut host DNA and kill them before you can do anything useful.
It would also be grate to have other enzymes (e.g. polymerase, polynucleotide kinase, phosphatase, DNA ligase. All of them required for serious cloning project).
I was thinking about scoring presence of an active "pigment gene" in the E.coli vs a gene with interrupted ORF. I didnt look in details about this gene and I wanted just to see if you have an idea how efficient it would be in E.coli for the purpose of cloning at first place.
How strong is signal/pigment/color in E. coli?
LacZ' is out there for ages. It is easy to get it but we can work together to make new and better things. You can demonstrate principles and in addition make a great tool.
If you can give me accession no. of your gene and sequence of the plasmid you are planing to use I can look into that. Im from Europe and I cant get involved physical but I think that I still can contribute.
Cheers, Srdjan
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