This here sounds great,
http://ijb.nigeb.ac.ir/index.php/ijb/article/view/276/165
Do E.Coli Promotors, ribosome bindingsites etc. match to B. subtilis?
Am Freitag, 13. Juli 2012 12:43:24 UTC+2 schrieb Mega:
Hallo,--
Has anyone heard of a plasmid for integration in the chromosome of b.subtilis?
If possible - that would be terrific - one that cuts out the antibiotic resistance afterwards.
I thought it would be a great idea to insert the lux genes into b.s. because
a) it's perhaps one of the most harmless bacteria.
b) it makes spores, so I can keep the petri dish when the sugars are gone, dry it and some months later streak it on a new agar plate - still glowing.
Where do you get such a vector from? Do you have to build it yourself or are there companies that sell it (just like pUC19, pVIB, pGreenII,...) ??
Thx
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