Hey, I've done something like this...
Took the pVIB plasmid (no need for a specific substrate! just oxygen + glucose) in E.Coli and put them into a marmelade jar containing LB medium (without ampicillin).
In the dark it was glowing much brighter than a petri dish with those bacteria (obviously).
It stopped glowing after a while (I think one week) perhaps because they have consumed all the sugar, or too much endotoxins?)
The best approach however would be to use B.Subtilis. They don't produce Endotoxins afaik. And they make spores, so you can just let them run out of sugar, wait for month, put sugar into it, and they'll grow and glow again!
And, of course, bacteria will lose those plasmids after some time. Best bet for an eternally glowing lamp is that you insert it into their chromosome. (Wanna do this ;) ) And cut out the marker gene (or take a marker that's not a clinical antibiotic; such as heavy metal resistance?), to avoid making resistant germs...
Am Sonntag, 22. Juli 2012 01:15:33 UTC+2 schrieb Chowe:
Hello! Im very new to DIYbio and I am looking to do an exciting first project. I work in a genetics lab so I have experience with most techniques. I have had many ideas for projects, that I happily found out most people have had the same ones too (glowing plants, glowing yogurt) but I thought a lava lamp would be the simplest way. I am wondering how you guys would go about completing this. I saw that cambridge igem team made a lava-lamp, seen here http://www.youtube.com/--watch?v=tUFscEVK5Ks I would like to make something just like that. I would prefer if I didn't have to induce it or if I did it would be through oxygen like the cambridge lava-lamp. It would be cool to have in my room to light it up at night. As with the glowing plants and yogurt they seem to be a lot harder and it seems like not many people have been successful so I wanted to choose a project that I would be more likely to succed. Thanks!Corey
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