You are lokking for the wrong name.
It's called pVIB. It's with an constitutive promotor, so no special medium required!
Am Mittwoch, 18. Juli 2012 03:03:15 UTC+2 schrieb Chuckles:
So I'm a junior, general biology major and I thought it'd be fun to get my own little lab going.--I want to do a simple little experiment to show to the nephews. I'd like to take some E. coli and make them glow by some good ol' fashioned bioluminescence using the pLux gene from Vibrio fischeri. I've done similar recombinant stuff in the lab at school and it's a super straight forward procedure so I thought it'd be fun.Trouble is... Where do I get the plasmids? I cannot find plasmids for the pLux gene anywhere. I thought about ordering a culture of V. fischeri and extracting the gene out via PCR... but I do not have a PCR machine yet. Hell, I don't even know if I can make a plasmid out of PCR products??Thanks for the help!
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