[DIYbio] Re: Finally a chance to create a glowing plant?

p.s - check this out: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0015461

On Friday, February 10, 2012 5:14:48 PM UTC+2, Mega wrote:

http://www.pgreen.ac.uk/a_pls_fr.htm - Agrobacterium disarmed Ti-
Plasmid
Kanamycin– Selektionsmarker ('nptI')
LacZ – Marker Blue-white screening


Here is a plasmid, as I understood this is a fully functional
Agrobacterium Ti-Plasmid.


It has a restriction site "SalI".
My pVIB has also SalI !!!

So ligating them together makes a plasmid that induces bioluminescense
in plants.

A question: The promotor works for agrobacterium and coli too?? Will
the bacteria glow too to mark them?
Because also the amp part of lux could be inserted and those will too
not make the X-Gal blue and be kanamycin resistant.
-> take glowing bacterium colony, culture them, plasmid preparation,
plasmid into void agrobacterium.
ready.


My plan is, going to a professor at mine, tell him and ask wheter I
could possibly use the lab and university as shiping adress.

Are my thoughts correct??


Yours

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