[DIYbio] Re: Free plasmids for amateur biologists.

Yes, even on this map isn't visible T7 promoter region in this plasmid. I was wrong what these plasmids are pBluScript derivatives - they actually are pCDNA3 plasmid derivatives. So, I analysed the plasmids sequence with help of UniPro Ugene and I've found, what between CMV promoter and genes coding HBsAg proteins is located T7 promoter, like in pCDNA3.

вторник, 3 июля 2012 г., 17:28:24 UTC+3 пользователь Dorif написал:

It definitevely won't be a crime, if I'll publish here a link to the map, what I received with the plasmids.) http://img341.imageshack.us/img341/512/mappwa.jpg

суббота, 30 июня 2012 г., 14:03:47 UTC+3 пользователь Dorif написал:

Hello! I have found the way to get some free plasmids with map and work protocol. The Aldevron company distributes free samples of pRc/CMV-Hbs(S) and pCMVHB-S2.S plasmids. Both are based on pBlueScript plasmid and contain Ampicilline resistance gene. Each sample contains 2-4 ug of plasmid DNA. Samples are delivered via postage service. Link to order samples: http://www.aldevron.com/products/dnas/plasmids/ 

I hope it'll be useful for somebody.

P.S.: sorry for my bad English.)

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