[DIYbio] Re: Free plasmids for amateur biologists.

So, at map all of us can see, what if we want want to removeHepatitis B genes from the plasmid, we can cut them off with help of XhoI, BamHI, KpnI and BssHII restriction enzymes. After this, instead of Hepatitis B genes we can insert something useful, replicate plasmid in E. coli, re-extract plasmid from them and implant needed gene to the target mammalian. Or even more: we cat cut off CMV promoter with NruI restriction enzyme and insert, for example GFP operon or something like that, to be expressed directly in bacteria.

вторник, 3 июля 2012 г., 17:28:24 UTC+3 пользователь Dorif написал:

It definitevely won't be a crime, if I'll publish here a link to the map, what I received with the plasmids.) http://img341.imageshack.us/img341/512/mappwa.jpg

суббота, 30 июня 2012 г., 14:03:47 UTC+3 пользователь Dorif написал:

Hello! I have found the way to get some free plasmids with map and work protocol. The Aldevron company distributes free samples of pRc/CMV-Hbs(S) and pCMVHB-S2.S plasmids. Both are based on pBlueScript plasmid and contain Ampicilline resistance gene. Each sample contains 2-4 ug of plasmid DNA. Samples are delivered via postage service. Link to order samples: http://www.aldevron.com/products/dnas/plasmids/ 

I hope it'll be useful for somebody.

P.S.: sorry for my bad English.)

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