I got 3 samples , two of them with some 209 ng/uL and 176.5 ng/uL and one experimental (didn't change the protocol for 1.5mL overnight culture to 3mL) with 54ng/uL.
So just double the ammount of chemicals when you make twice the volume ;D And get much better yields. The miniprep was done with just 3000 rpm for all and therefore longer peroids of rotation, I have to get a better device...
Am Donnerstag, 12. Juli 2012 10:39:38 UTC+2 schrieb Mega:
--Here is a plastic bottle that says TE-EP redissolving buffer. This should be Tris EDTA?I'm going to ask if I can have a few milliliters of it.
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