Re: [DIYbio] Bacillus Subtilis integration vector

On Fri, Jul 13, 2012 at 6:43 AM, Mega <masterstorm123@gmail.com> wrote:
> Hallo,
>
> Has anyone heard of a plasmid for integration in the chromosome of
> b.subtilis?

a quick google of "subtilis homologous vector integration" comes up
with loads of answers
www.bgsc.org/Catpart4.pdf
www.bgsc.org/NewProducts/Middleton.pdf

and something a little closer to home:
http://partsregistry.org/Bacillus_subtilis
"Integration plasmids is the most used technique by specialist in B.
subtilis. They are E. Coli plasmids with two homologous sequence with
two fragments of a chromosomal gene of B. Subtilis, and a resistance
cassette inserted in between. If your brick is cloned in between these
two homologous region, the vector act as a shuttle that integrate your
construct into the chromosome."


--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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