Re: [DIYbio] check LAMP! pcr is soooooo 90ies..

The papers I've seen on LAMP look very promissing. Especially this one on malaria: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0013733

I'd like to read some more about the inhibitory effects that have been described when samples are not completely purified. I can imagine that 6 primers make the reaction more sensitive to polutants. Also the economic performance of the method is unclear to me (e.g. how much does it cost?).

Does anyone have a good reference to an article where these parameters are studied? I'd love to learn more about it!

On Monday, 2 July 2012 15:22:14 UTC+2, Cathal wrote:

LAMP is an awesome method for on-site PCR, but (speaking as someone who
*hasn't* tried it) seems to be harder to optimise and design for
general-purpose DNA amplification.

That is, standard PCR is better for prototyping a reaction, but for
creating a protocol for general or regular use, LAMP is probably a good
next step; as you point out, it requires little in the way of
instrumentation, and because there's no cycling of temperature, that's a
huge source of experimental error removed! Awesome for walk-by workshops
for DNA testing etc..

On 02/07/12 10:59, Marc Dusseiller wrote:
> hei garage biologists,
>
> just saw some interesting low-cost stuff at a conference in kenya. you know
> about LAMP? seems like a great and cheap alternative to pcr, especially on
> the instrumentation side.
>
> http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0000147
>
> more stuff from low-cost diagnostics soon,
> marc
>


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