> Also how exactly would you build a plasmid that would insert part of its genes into the chromosome, any certain plasmids need to be used?
http://www.bgsc.org/Catpart4.pdf
Here are some plasmids listed. It is written that on request, they may send you a plasmid for free if you use it for non-profit things.
Well, the plasmid needs two flanking sites that fit to a gene in the Bac. Subt. Chromosome. Then in 1 out of 10000 cases, homologous recombination occurs. The plasmid does not replicate, so if the bacillus doesn't die from the antibiotic and forms a colony, it has the gene casette integrated.
In theory, you could also use pUC19, fit in flanking sites of B.Subt. (where to get the template?, can you design the primers?), inside the flanking sites Lux operon + Resistance , amplify it in E.coli, transform B.S.
> I will have full time access to my schools laboratory.
Awsome ;)
> For that project would you recommend B.Subtilis?
Yeah, because of the spore formation. E.Coli will die on the plates after a while when the sugar is out. After at last one year, you cannot revive them again.
The spores of B.S., when they 'smell' better conditions, will 'wake up' again and in theory, still after 50 years of dryness, etc. you'll get a glowing B.Subt. again.
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