The result is a continuous loop structure: http://www.youtube.com/watch?v=5Wi-kkSFy48
The patent situation is also what I see as the main limitation for it to be applied as a true POC diagnostics method. I also think the need for purification of the DNA, either by qiagen kits or boiling and centrifugation is a major drawback.
There have been studies before on how to modify the polymerase in such a way that it still works regardless of common PCR inhibitors: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655666/. I assume the Bst Polymerase has not been designed in the same way. Also NE Biolabs does not make any statement on inhibitors on the FAQ site: http://www.neb.com/nebecomm/products/faqproductM0537.asp
Reducing the amount of steps of the procedure by boiling the sample including the reagents is also not possible, since the Bst Polymerase becomes heat-inactivated at temperatures greater than 72 C...
On Wednesday, 4 July 2012 08:39:22 UTC+2, Nathan McCorkle wrote:
On Wed, Jul 4, 2012 at 1:50 AM, Ravi <sheth.u.ravi@gmail.com> wrote:--
> 2 problems:
> 1) everything remotely connected to LAMP is heavily patented. Specific
> assays, reporting mechanisms, devices etc.
> 2) cannot replace PCR as you are amplifying a "chain" DNA structure, not
a chain structure???
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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