> the genes might get silenced by methylation.. etc.
Ok, But that can depend on the plant?? If I (actually, my agrobacterium) put it into a willow, it may get silenced. Then I put it into a maple, and it may work?
And: the chemicals needed (same chemicals as in bacteria) will be produced? In what quantities we don't know. Clearly.
>Further, you don't know whether it will glow brightly enough to be visible.
This risk I am willing to take. ;)
You could add aldehydes (such as decanal) to the water you 'feed' it with. Because the aldehydes will be the limiting factor.
Then it will glow brighter. If the luciferin works (which sould work anyway).
2012/7/13 Cathal Garvey <cathalgarvey@gmail.com>
> so I have toHere's your problem. There is _no_ way to know whether it will work as
> be sure it works.
intended until you try it.
Biology's complex; while we can often make pretty good predictions about
qualitative stuff: "will it glow, or will it not glow?".. we're pretty
much in the dark on the quantitative stuff: "How much will it glow? Will
it even be perceptible?"
You have two problems; without someone else doing it first, you don't
know if it'll work _at all_: the proteins might not end up in the
correct cell compartments, the precursors might get depleted too
quickly, the genes might get silenced by methylation.. etc.
Further, you don't know whether it will glow brightly enough to be visible.
So, if you wanna do it (it's ambitious, it's awesome!), be prepared to
not know if it'll work.
www.indiebiotech.com
On 13/07/12 21:05, Mega wrote:
> Once again, this old topic :D
>
> I'm gonna get that DNA synthesized, but first I have to go haunting for
> fundings.... If you say it will work. It will cost some 3800$ so I have to
> be sure it works.
>
>
>
> The bacterial luciferin-luciferase system per se should work in plants.
> (Why not?)
>
> If I have it synthesized like this: best viral Plant promotor known - RBS -
> LuxA (codon usage modified!) - Terminator --- same for Lux B,C,D,E....
> it will definitely create the substances needed to glow in planta???
>
> The company will insert it into any vector, so I choose an agrobacterium
> Ti-Plasmid.
>
> Insert Plasmids (binary system) into agrobacterium. Put it on plant seemen.
> Goal - a glowing plant??
>
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