Re: [DIYbio] Re: Free plasmids for amateur biologists.

May be cooker won't destroy the DNA, but hot alkaline solution, used for hydrolysis of some organic compounds(I study at the Faculty of Chemistry, and my profile is Organic Chemistry, we use hydrolysis reactions to remove protector groups) - will.) And, fortunately, Moldova isn't a part of European Union at now, so, I don't need any permissions(I've read our laws).)

понедельник, 2 июля 2012 г., 18:31:00 UTC+3 пользователь Cathal написал:

Kill what in the pressure cooker? DNA will happily survive pressure
cooker temperatures, although you might deaminate a few thymines here
and there.

The risks of handling this DNA aren't large, unless you snort it
(plasmid DNA can apparently be delivered in tiny amounts to nasal cells
in this manner, go figure). The antigen is probably the one used for
vaccination, but you don't want to express that in your own cells, for
fear of generating a more generalised autoimmune response.

As to putting it into other cells, depending on your region this may be
illegal: Hepatitis is probably on the US select organisms list, for
example, which puts work with Hepatitis DNA squarely in the "possibility
of having house stormed by angry unsympathetic soldiers" bracket.

In the EU, as with anything else you'd need a risk assessment of the
gene cassette and permission from your local GMO-regulating "competent
authority": if you can convince them that the protein doesn't carry the
infectious risks of the organism of origin, they might allow it as a
Class 1 Containment GMM, meaning you can work with it in a licensed
Class 1 lab. Don't even daydream of getting Heptatitis DNA deregulated:
it's already nigh-impossible to get them to deregulate things like GFP
without *all* the money.

On 02/07/12 16:21, Mega wrote:
> Oh, really... You're right, it encodes a hepatitis surface protein.
>
> At first I just read "pBluescript for free" and was thrilled about it...
>
> (But given the plasmid map and that the gene of interest is within the
> needed restriction sites, you could just cut it out and kill it in the
> pressure cooker. )
>
>
>
> Am Samstag, 30. Juni 2012 13:03:47 UTC+2 schrieb Dorif:
>>
>> Hello! I have found the way to get some free plasmids with map and work
>> protocol. The Aldevron company distributes free samples of pRc/CMV-Hbs(S)
>> and pCMVHB-S2.S plasmids. Both are based on pBlueScript plasmid and contain
>> Ampicilline resistance gene. Each sample contains 2-4 ug of plasmid DNA.
>> Samples are delivered via postage service. Link to order samples:
>> http://www.aldevron.com/products/dnas/plasmids/
>>
>> I hope it'll be useful for somebody.
>>
>> P.S.: sorry for my bad English.)
>>
>

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