Yeah, there was a 260:2801 ratio...
I didn't write it down, but it is still saved in the computer .... Gonna get that.
Did a transformation with one of those plamid (176 ng/uL), gotta look if it works and how many colonies... (Inkuating them at room temp so still no visible growth...)
2012/7/17 Nathan McCorkle <nmz787@gmail.com>
The first two concentrations are decent, did you records the 230, 260, and 280nm absorbance levels? The ratios between them tell you about organic solvent contamination (230nm, mainly phenol but others too) and DNA to protein level which is a measure of general purity (280nm is tryptophan's peak, which is used as the average/common protein peak)
On Jul 17, 2012 5:23 AM, "Mega" <masterstorm123@gmail.com> wrote:--I got 3 samples , two of them with some 209 ng/uL and 176.5 ng/uL and one experimental (didn't change the protocol for 1.5mL overnight culture to 3mL) with 54ng/uL.
So just double the ammount of chemicals when you make twice the volume ;D And get much better yields. The miniprep was done with just 3000 rpm for all and therefore longer peroids of rotation, I have to get a better device...
Am Donnerstag, 12. Juli 2012 10:39:38 UTC+2 schrieb Mega:--Here is a plastic bottle that says TE-EP redissolving buffer. This should be Tris EDTA?I'm going to ask if I can have a few milliliters of it.
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