Re: [DIYbio] Re: A simple project for PCR first-timers

> Forgive me if this is foolish, but does the Y within the primer sequence
> correspond to thymine or cytosine?

Yeah, the Y means a mixture of C and T. Most primer manufacturers
allow you to mix bases. Comes in handy sometimes.


> If so, will the primers contain
> a mixture of each sequence??

Correct. Ideally there will be two different oligos in equal proportions.


-cory

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