Makes sense! Thanks!
Have you made any progress with step 4?
On Saturday, July 14, 2012 2:57:45 AM UTC-4, cory....@gmail.com wrote:
-- On Saturday, July 14, 2012 2:57:45 AM UTC-4, cory....@gmail.com wrote:
> Forgive me if this is foolish, but does the Y within the primer sequence
> correspond to thymine or cytosine?
Yeah, the Y means a mixture of C and T. Most primer manufacturers
allow you to mix bases. Comes in handy sometimes.
> If so, will the primers contain
> a mixture of each sequence??
Correct. Ideally there will be two different oligos in equal proportions.
-cory
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/vuCOWcsO2AIJ.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment