Hello, I have been a follower of Helicos for a few years now. My question is to your technical expertise. Do you see this platform competitive in the current market or is it a league of its own because tSMS and DRS and lack of amplification making a direct threat to the staus quo?
Thanks for your time
On Thursday, August 23, 2012 5:00:29 PM UTC+2, Nathan McCorkle wrote:
-- On Thursday, August 23, 2012 5:00:29 PM UTC+2, Nathan McCorkle wrote:
This video says they take sheared ssDNA, add polyA tail, hybridize
about 1 molecule per square micron on a surface coated with polyT,
then add polymerase and a single species of fluorescent nucleotide,
rinse, illuminate with laser light and scan the surface with a camera,
cleave fluorophore, then repeat with a different species of nucleotide
and more polymerase.
http://www.helicosbio.com/Portals/0/Videos/tSMS-How_It_ Works.flv
I wonder if they're using a single pixel camera with a PMT backend?
I wonder if I could replicate with just 1 pixel (1 molecule only), I
could use this to provide feedback for closed-loop DNA synthesis
operations.
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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