Re: [DIYbio] 3D printing medical devices

Here's a good view of our printer, after we had first gotten it to print (in ink):

https://secure.flickr.com/photos/68942898@N05/7175637623/in/pool-bioprinter

The grey rectangle on top is one of the linear actuators we scavenged out of the CD drives. It's got a little stepper motor with a worm gear, moving the CD read head back and forth. The inkjet cartridge is mounted on the read head. We had to cut out some of the grey frame to allow the cartridge to move the full distance (only 2 inch or so - the radius of a CD).

There's another one of these linear actuators on the bottom, at a 90 degree angle. The printing platform (in this case, a post-it note) sits on top of the read head there. Only the read heads and what's attached to them are moving: the bottom head is moving front to back with the post-it, the top head is moving left to right with the print head.

We'll have some better pics for you guys later - hoping to put together an Instructable on this soon.

Patrik

On Sunday, September 30, 2012 1:08:29 AM UTC-7, wgh...@gmail.com wrote:

Hi Again,

Its like drugs to me to hear about this project.  I appreciate your continued assistance in helping me understand more about your project.  In the XY configuration, where was the inkjet cartridge, was it attached in such a way that was independent of the printer?  I can't explain why I want to do this, but I just do :>  I will keep asking questions until you won't let me anymore or they kick me off the list :>

-Tim

On Fri, Sep 28, 2012 at 12:28 AM, Patrik D'haeseleer <pat...@gmail.com> wrote:
Heh - the food coloring (and the arabinose) was just with an old inkjet printer we rescued from the sidewalk. Cut off the top of the cartridge, rinse out thoroughly, fill with water + food coloring, print test page.

For our second generation bioprinter, we built an XY-platform from the read head mechanism from two old CD drives (need to search around for the type that uses a stepper motor), driven by an Arduino. This part was entirely inspired by Hackteria's work along these lines:

http://hackteria.org/wiki/index.php/HackteriaLab_2011_Commons#Micro_Manipulator

http://hackteria.org/wiki/index.php/DIY_Micro_Dispensing_and_Bio_Printing

http://hackteria.org/wiki/index.php/DIY_Micro_Laser_Cutter

http://hackteria.org/wiki/index.php/DIY_Microfluidics#Advanced_DIY_Microfluidics

http://hackteria.org/?p=1186

And I think they got their idea from these guys:

http://builders.reprap.org/2010/08/selective-laser-sintering-part-8.html




On Friday, September 28, 2012 2:12:52 AM UTC-7, wgh...@gmail.com wrote:
Wow, this is the most real info on bio printing I have ever come across thanks!  I have a couple more quick questions for you.  When you used the food coloring did you just print it onto paper?  What are you using for x-y positioning of the printer head?  Are you printing to a well or what are you actually printing on?  Somehow you ended up with something in a petri dish but I am confused how you got it in there.  Can you please elaborate?  I am quite excited by your progress.

-Tim

P.S.

The main issue as I understand it is positioning.  It sounds like your using cd motors of some kind to solve this issue.  Can you elaborate on how your solving this issue?  What is the printing resolution needed for diybio cell printing in your opinion?  Is 96 dpi too much or not enough etc...?  Makerbot appears to have solved much of the issues your probably facing, have you looked at adapting that platform to bio printing just curious?

On Thu, Sep 27, 2012 at 9:43 PM, Patrik D'haeseleer <pat...@gmail.com> wrote:


On Thursday, September 27, 2012 3:40:56 PM UTC-7, wgh...@gmail.com wrote:

I have been very interested in doing bio printing.  I tried to get an old hp deskjet printer and make it print a jello like substance but couldn't even do that.

Have you tried just printing with food coloring in water? That is the first thing we did, and that worked fine. One problem you may be having with a gel is that the inkjet head expects a fluid of a certain viscosity. Plus as the thermal inkjet vaporizes some of the ink, some gelatin may burn and eventually clog the print head.

The second thing we tried, after food coloring, was to print with arabinose in water onto filter paper. Then we put the filter paper onto a lawn of E. coli with the pGLO plasmid containing GFP under an arabinose inducible promoter, so wherever we had printed arabinose, we saw the GFP light up:

https://secure.flickr.com/photos/68942898@N05/6799091190/in/pool-bioprinter

We clearly got a lot of diffusion of the arabinose, but you can still make out the BioCurious logo lighting up in GFP.

Cells suspended in liquid should be feasible using an inkjet, but if you really want to print with cells embedded in a gel, you'll probably want to move to a syringe pump system. We're currently looking at mounting a DIY syringe pump (probably driven by another linear actuator from a CD drive) on our BioPrinter.
 
 I am completely frustrated with how to do this.  Can anyone give me some real help to figure out how to do this for a diybio person?  I am trying to think of what would be a good approach to developing the diybio bio printer.  My idea was that I would start with trying to print a jello like gel with different colors in the wells to "test" that it is working.  After I complete that task I would then move on to perhaps printing some plant cells or something.  I would then like to print cells that are not dangerous to work with like human cells.  I am curious are bone cells considered safe?  What cells are appropriate for bio printing that would be safe?  I keep looking at the makerbot and thinking in my mind, can't this be adapted to bio printing?  The only thing I see missing is that the platform needs to move up and down that is being printed on.  Isn't that all that is missing?  I guess the resolution may not be high enough either.  Anyway, I would love to get a simple working bio printer up and working, help me ob1, YOUR MY ONLY HOPE! :>

-Tim

P.S.

I turn cell phones into robots.  I am very familiar with arduinos, laser printing, serial communication.  You can see my latest robot here:

http://www.youtube.com/watch?v=E_q5WD3dTkE

On Wed, Sep 26, 2012 at 12:12 PM, kingjacob <king...@gmail.com> wrote:
You can print in titanium using Direct Metal Laser Sintering. You can pretty much print with any material you can turn into a powder or resin. A friend of mine (papers below) even used a NaCl mixture to 3D print tissue scaffolding.

Computer-aided tissue engineering: benefiting from the control over scaffold micro-architecture.

http://www.ncbi.nlm.nih.gov/pubmed/22692601

Scaffold pore space modulation through intelligent design of dissolvable microparticles.


Computer-aided tissue engineering of a human vertebral body.


On Wed, Sep 26, 2012 at 4:45 PM, Nathan McCorkle <nmz...@gmail.com> wrote:
I've never heard anyone on here discussing printable hip
replacements... it sounds like a bad idea anyway, seeing as how poor
3D printer plastics fair in strength. Most joint replacements are made
of titanium, etc... Who put that in there?

It also has really old info scattered throughout, and doesn't mention
anything of the years of FBI interaction we've had

On Tue, Sep 25, 2012 at 9:02 PM, Bryan Bishop <kan...@gmail.com> wrote:
> The wikipedia articles are still awful.
>
> http://en.wikipedia.org/wiki/Biohacking
> http://en.wikipedia.org/wiki/DIYbio
> http://en.wikipedia.org/wiki/Biopunk
>
> At least that last one is somewhat less awful. Anyone want to take cleanup
> duty?
>
> - Bryan
> http://heybryan.org/
> 1 512 203 0507
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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--
Cheers,
Jacob Shiach
editor-in-chief: Citizen Science Quarterly
twitter: @jacobshiach

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