Cathal,
as I got no answer from the patent-holder I asked the lab whether they'd have one without the patented marker, be it marker-free or any other marker. Negative.
(
Anyway, I don't really like resistances other than kanamycin (but I'd have taken the plasmid for sure!) ;) The (unlikely) escape of kanamycin genes into nature wouldn't affect the environment as kR is wide-spread in most soil bacteria. Barely used therapeutically in humans any more today.
)
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