Re: [DIYbio] Re: Looking for Chloroplast integration Vector

Well, I don't know how useful they are in plant engineering, but I've
looked at medically unimportent *bacteriocins* in the past as potential
selective agents. There are loads of bacteriocins (peptide antibiotics)
that are unused because they might trigger allergies, and because they
can't be orally taken as medicines (peptides get digested!).
Lantibiotics are another avenue here; some of them are medically
interesting, but others are likely to be dead-ends, and might be
penetrative enough to use with plants if they affect chloroplasts.

That's the biggie though; do they affect chloroplasts? I don't think
anyone tests Bacteriocins for that sort of thing! Perhaps look through
the bacteriocin databases for ones known to affect species that are
affected by kanamycin, and work from there; do they affect cell wall
synthesis? Then check if chloroplasts have similar cell walls to the
affected species. Do they affect protein translation? Compare the
chloroplast ribosomes to affected species and make a guess.

Wild west territory, this is. :)

On 27/09/12 11:44, Andreas Sturm wrote:
> Cathal,
> as I got no answer from the patent-holder I asked the lab whether they'd
> have one without the patented marker, be it marker-free or any other
> marker. Negative.
>
>
> (
> Anyway, I don't really like resistances other than kanamycin (but I'd have
> taken the plasmid for sure!) ;) The (unlikely) escape of kanamycin genes
> into nature wouldn't affect the environment as kR is wide-spread in most
> soil bacteria. Barely used therapeutically in humans any more today.
> )
>


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