Well, that sounds great.
If you want to make something glowing (there's actually no luminescent protein, but a pathway that makes light by oxidation of aldehydes), you can get a plasmid called pVIB from Carolina Bio for 10$. Insert it into bacteria (optimized for E.Coli), and they will glow.
Here you can find fotos of my first try, with pVIB
http://diyspartanbiotech.wordpress.com/2012/05/11/transforming-e-coli-with-pvib-plasmid/
When I plated them on new, fresh selection plates they were much brighter, it truely was enlightening!
On Thu, Sep 6, 2012 at 12:55 AM, HadyE <hady629@hotmail.com> wrote:
I was actually hoping to just do a basic experiment to make a luminescent protein in a vial, just to see if my technique is correct. I'll be searching, but if anyone has any pointers or experience, please, by all means let us know.
Hady--To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/vdgBlgkn2tEJ.
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