Ok,
Thank you!! If the termination doesn't work you'll get fusion proteins thus. If you place the other protein in frame, of course. Great.
On Fri, Sep 28, 2012 at 12:45 PM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
I think it mainly gives you a more reliable termination. Terminators are always "leaky" to some extent. Putting two in a row guarantees you won't get read-through into the next gene downstream, which could cause all sorts of crosstalk in whatever genetic circuit you're designing.To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/4e9ctWqDRrcJ.
On Friday, September 28, 2012 2:25:30 AM UTC-7, Mega wrote:--Hi,I saw that IGEM uses a double terminator sometimes. Does that give you better protein expression?
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