I like GelGreen. Cheaper than SybrSafe, supposedly just as safe and brighter.
There is some autofluoresence when the dye is in solution but it has a different peak than when it binds to DNA. You can fool around with qPCR-lite by adding the dye to a PCR mix and checking fluorescence after each step.
Standards are necessary - but can they be treated sequence independent?
Mac
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+1.231.313.9062 / mac@diybio.org / @100ideas
-- On Wed, Sep 5, 2012 at 1:52 PM, Dakota <dkotes@gmail.com> wrote:
Meant to add this just as an interesting thing to read about or watch the videon on. SNAP-Tag from NEB. Really cool flourscent labelling system with fusion protein and a DNA repair enzyme. It was supposed to take over for GFP but I guess it hasn't caught on as much as they thought it would. A little unrelated but, neat nonetheless--
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+1.231.313.9062 / mac@diybio.org / @100ideas
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