[DIYbio] Re: DIY chemostat

It only becomes cheaper to build your own complex equipment if you have the time and skill set, otherwise you are going to have to hire people or most likely have to buy extra equipment for the build. Though if you have the skills or some smart friends DIY equipment can be far cheaper. 


As for maintaining a genetically pure stock it would be best to go back to an original slant or glycerol stock of the parent strain every month or so, propagation reactor or turbido/chemostat. 

On Sunday, November 18, 2012 2:13:01 AM UTC-5, AdvancedMudPieMaker wrote:
Patrick,

Thank you for that link.  Looked for pricing online and could not find it so I will call on Monday.

I have concluded that yeast propagation equipment would better match my needs because of genetic drift in the chemostat.

Here the DIY challenge is sterility and oxygenation as the scale increases.  Commercial equipment is excellent.  Can 200 L and the stair steps leading up to that be done in a DIY manner at lower cost?

Thanks to all who posted.

Alan

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