Thanks for everyone's input! Sounds like agar plates would be the way to go.
Xabier, I will look and see if I have any of those chemicals available in our lab, otherwise I can get them from the university.
-Conner
On Saturday, November 17, 2012 9:37:21 AM UTC-5, poli wrote:
Yes antibiotics can be used to get a new culture but I wouldn't do it in the liquid phase. Make F/2, TAP, or whatever media you're using agar plates with antibiotics. Spread your culture on it and wait for single colonies. Aseptically transfer a single colony to new sterile media.
On Friday, November 16, 2012 11:27:17 AM UTC-5, Conner Berthold wrote:Hi,Was wondering if anyone had tried to use antibiotics to make bacteria free, axenic algal cultures? If found this paper on it, and the main problem looks like the need for a mixed antibiotic mixture to effectively remove the bacteria.-Conner
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