Hm - wonder whether that would work for our dinoflagellates. I've never heard of growing them on a solid medium but I have to admit I haven't really looked into how they were originally isolated. They do replicate very slowly - every ~5 days - so we'd have to grow them for a couple of weeks on agar before they would form noticeable colonies. Not sure they would be very happy with that.
Patrik
On Saturday, November 17, 2012 6:37:21 AM UTC-8, poli wrote:
Yes antibiotics can be used to get a new culture but I wouldn't do it in the liquid phase. Make F/2, TAP, or whatever media you're using agar plates with antibiotics. Spread your culture on it and wait for single colonies. Aseptically transfer a single colony to new sterile media.--
On Friday, November 16, 2012 11:27:17 AM UTC-5, Conner Berthold wrote:Hi,Was wondering if anyone had tried to use antibiotics to make bacteria free, axenic algal cultures? If found this paper on it, and the main problem looks like the need for a mixed antibiotic mixture to effectively remove the bacteria.-Conner
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