The fungus is underneath the nail.
Taking a culture involves cutting through the nail to reach it.
Not something you want to do routinely.
I would expect a profile from seven or eight different wavelengths to be pretty good
at distinguishing fungus from bacteria and pure scattering (which would affect all wavelengths
to much the same degree, i.e. no absorbance).
If two of the wavelengths are in the infrared and one is in the ultraviolet, it is possible that
the detector would be more sensitive and reliable than the human eye. I have made chlorophyll
detectors that can tell real plants from fake, even when the human eye cannot. And they only used
four LED colors (one in the infrared).
Another benefit is that the UV LED could be used as a treatment, as well as a detector.
:-)
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Get a free science project every week! "http://scitoys.com/newsletter.html"On Thu, Nov 22, 2012 at 11:00 AM, Josiah Zayner <josiah.zayner@gmail.com> wrote:
Realistically I think the biggest problem is not reading data using light from through or from a nail, they do this all the time with O2 sensors. The biggest problem is _how_ do you differentiate an infected nail from a non-infected one. Perhaps you say differences in absorbance, but every single nail will have differences in scatter/absorbance. It would be easy to detect serious infections but then again those can be seen by the eye.What specific properties of small quantities of fungus do you think will give it a distinct scatter/absorbance as say compared to dirt, bacteria, yeast or psoriasis?
PCR is cheap easy and can detect fungus on a low level. So can just taking a swab and looking at it under a microscope or culturing the fungus. What makes an photometric detection method better?Fast and reliable PCR/sequencing/RFLP assay for identification of fungi in onychomycoses
http://jmm.sgmjournals.org/content/55/9/1211.full
On Wednesday, November 21, 2012 5:52:43 PM UTC-6, Simon Field wrote:I would bet that a wideband photodetector surrounded by some different colored LEDs(infrared, red, yellow, green, blue, violet, ultraviolet) and placed against the nail wouldgive you plenty of data to make the decision. Pot them in black silicone so the sensoronly sees the reflected and scattered light (not direct light from the LEDs), and thenswitch each LED on for a tenth of a second while reading the sensor output. Now you haveseven channels of data, and you can calibrate it against known healthy and infected nails.On Wed, Nov 21, 2012 at 3:09 PM, wolass <wol...@gmail.com> wrote:Well, I thought simply culture would be enough to determine the strain. PCR is rarely used in clinical practice in Poland, so every physician relies on mycological diagnosis.
Thanks for your help.
W dniu środa, 21 listopada 2012 19:58:39 UTC+1 użytkownik Nathan McCorkle napisał:
> Would trying to PCR then sequence fingernail clipping DNA work? You could design an assay using 28S rRNA or something like that to determine what species are present? I don't know if that's the kind of info you want.
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> Sequencing and Analysis of Fungal rRNA Operons for Development of Broad-Range Fungal PCR Assays
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> On Wed, Nov 21, 2012 at 10:41 AM, wolass <wol...@gmail.com> wrote:
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> I want to investigate wether there is a difference in wavelenghts absorbed by healthy nails and nais infected with pathogenic fungi.
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> Nathan - nailbed is my main interest as this is where fungi grow. I havent thought of your method, but it is worth investigating. Thank You!!!
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> I will also have to look if there is any fiber based spectrometer lying around :)
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> Yes i do not want tissue denaturation - this is why i did't want the KOH method, as specific fungal strains may give different wavelength absobance.
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> W dniu środa, 21 listopada 2012 18:00:57 UTC+1 użytkownik Nathan McCorkle napisał:
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> > with a fiber-optic based spectrometer, you could just do backscatter sampling, where a single fiber has light coming out, hit's your sample, and what is reflected back enters the same fiber then splits off to the detector... you could be getting nailbed data that way though.
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> > KOH might work to dissolve things, but it could also cause bands to shift or appear/disappear completely as you'd be denaturing some proteins (at least), you could also try meat tenderizer (or fresh pineapple juice or papaya seed extract) for the protease activity
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> > --
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> > Learn more at www.diybio.org
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> > --
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> > -Nathan
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