with a fiber-optic based spectrometer, you could just do backscatter sampling, where a single fiber has light coming out, hit's your sample, and what is reflected back enters the same fiber then splits off to the detector... you could be getting nailbed data that way though.
KOH might work to dissolve things, but it could also cause bands to shift or appear/disappear completely as you'd be denaturing some proteins (at least), you could also try meat tenderizer (or fresh pineapple juice or papaya seed extract) for the protease activity
here are some somewhat looking relevant papers
(ctrl-f nail here, there are some images at the end) http://spx.arizona.edu/Theses/Lovisa_Thesis.pdf
On Wed, Nov 21, 2012 at 7:52 AM, wolass <wolass@gmail.com> wrote:
Hey guysI would like to measure what wavelengths are absorbed by the human nail plate.My only idea so far is to take the nail clippings, dissolve them in KOH and than pour this liquid sample into a spetrophotometer.Do you see other options?I will be grateful for your help(I'm a medical student with main field of interest in dermatology)--
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