Re: [DIYbio] Methodology help needed - how to measure nail light absorbance

Wow Simon, that is something new, what actually could be done with little money investment. I like the idea, although i'm not shure if it would be that accurate. I guess we have to build it and see.

W dniu czwartek, 22 listopada 2012 00:52:43 UTC+1 użytkownik Simon Field napisał:
> I would bet that a wideband photodetector surrounded by some different colored LEDs
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> give you plenty of data to make the decision. Pot them in black silicone so the sensor
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> switch each LED on for a tenth of a second while reading the sensor output. Now you have
> seven channels of data, and you can calibrate it against known healthy and infected nails.
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> -----Get a free science project every week! "http://scitoys.com/newsletter.html"
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> On Wed, Nov 21, 2012 at 3:09 PM, wolass <wol...@gmail.com> wrote:
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> Well, I thought simply culture would be enough to determine the strain. PCR is rarely used in clinical practice in Poland, so every physician relies on mycological diagnosis.
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> Thanks for your help.
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>  W dniu środa, 21 listopada 2012 19:58:39 UTC+1 użytkownik Nathan McCorkle napisał:
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> > Would trying to PCR then sequence fingernail clipping DNA work? You could design an assay using 28S rRNA or something like that to determine what species are present? I don't know if that's the kind of info you want.
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> > Sequencing and Analysis of Fungal rRNA Operons for Development of Broad-Range Fungal PCR Assays
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> > Prasanna D. Khot, Daisy L. Ko, and David N. Fredricks
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> > On Wed, Nov 21, 2012 at 10:41 AM, wolass <wol...@gmail.com> wrote:
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> > I want to investigate wether there is a difference in wavelenghts absorbed by healthy nails and nais infected with pathogenic fungi.
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> > I am also trying to figure out an artificial nail model to use in onychomycosis trials.
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> > Nathan - nailbed is my main interest as this is where fungi grow. I havent thought of your method, but it is worth investigating. Thank You!!!
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> > I will also have to look if there is any fiber based spectrometer lying around :)
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> > Yes i do not want tissue denaturation - this is why i did't want the KOH method, as specific fungal strains may give different wavelength absobance.
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> > W dniu środa, 21 listopada 2012 18:00:57 UTC+1 użytkownik Nathan McCorkle napisał:
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> > > with a fiber-optic based spectrometer, you could just do backscatter sampling, where a single fiber has light coming out, hit's your sample, and what is reflected back enters the same fiber then splits off to the detector... you could be getting nailbed data that way though.
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> > > KOH might work to dissolve things, but it could also cause bands to shift or appear/disappear completely as you'd be denaturing some proteins (at least), you could also try meat tenderizer (or fresh pineapple juice or papaya seed extract) for the protease activity
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> > > here are some somewhat looking relevant papers
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> > > http://www.google.com/patents?hl=en&lr=&vid=USPAT6631199&id=pCoOAAAAEBAJ&oi=fnd&dq=backscatter+spectroscopy+fiber+nailbed+keratin&printsec=abstract#v=onepage&q&f=false
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> > > http://www.google.com/patents?hl=en&lr=&vid=USPAT8145286&id=tc4KAgAAEBAJ&oi=fnd&dq=backscatter+spectroscopy+fiber+nailbed+keratin&printsec=abstract#v=onepage&q&f=false
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> > > (ctrl-f nail here, there are some images at the end) http://spx.arizona.edu/Theses/Lovisa_Thesis.pdf
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> > > On Wed, Nov 21, 2012 at 7:52 AM, wolass <wol...@gmail.com> wrote:
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> > > Hey guys
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> > > I would like to measure what wavelengths are absorbed by the human nail plate.
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> > > My only idea so far is to take the nail clippings, dissolve them in KOH and than pour this liquid sample into a spetrophotometer.
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> > > Do you see other options? 
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> > > I will be grateful for your help 
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> > > (I'm a medical student with main field of interest in dermatology)  
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> > > --
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> > > Learn more at www.diybio.org
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> > > --
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> > > -Nathan
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