Re: [DIYbio] Protoplast isolation succesful ;)

Hi,

As the pectinase had finally arrived, I mixed some cellulase (around 10-20 uL) with pektinase (just some with a spatula)  in an 1.5 mL eppi. Then I added 8% xylitol solution (isotonic, that the cells don't burst from osmotic stress. I had no manitol like the instruction video said so I had to use that).


Incubated for ca 90 minutes, and then microscoped some of it. Find the pictures attached ;)


I think I will let the enzymes work over night with the rest, and then centrifuge it, discard supernatant, add xylitol solution. And microscope again.


Does anyone know if there are some protoplasts in it? Highest zoom = 400

I belive to see there are some cells of which you see the chloroplasts. But the ''chloroplasts'' might be as well be small entire plant cells in a sugar coating? 

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/X-2XilIHEpsJ.
For more options, visit https://groups.google.com/groups/opt_out.