Re: [DIYbio] Re: 7 days until Primer Day - primers for a penny

Well, it went pretty well for me-- I got some degenerate primers, and a few that were over 30bp.

For those are interested in what I got:

I ordered two pairs of microsatellite primers reported for Taraxacum officinale (common dandelion) in (2) and successfully used in multiplex PCR in (1), modified with M13(-21) tails as described in (3). That's the dandelion part; now I just lack corresponding M13(-21) oligos with fluorescent tags, and if I somehow manage to get those, I could maybe do some multiplex PCR on dandelion microsatellites and get a fragment analysis from the University of New Mexico.

On the Triops front, I got the COI primers reported from (4), the degenerate primers used to amplify the extant snippet of caudal from T. longicaudatus (reported in a dissertation), and the adaptor primers used with XhoI in (5). Unfortunately, I discovered that I don't have any way to get DNA out of my T. cancriformis specimen after all, so I'm in a jam, because that's my last non-preserved specimen and it'll take two months to get another cohort of critters. I've got to fix this situation as soon as possible.

There was no way I could possibly have afforded this without the Primer Day offer, and I'm very glad I caught word of it.

--Tristan


1. Falque et al. Development and characterization of microsatellite markers in the sexual-apomictic complex Taraxacum officinale (dandelion). Theoretical And Applied Genetics (1998) vol. 97 (1-2) pp. 283-292

2. Matsumoto et al. Alien Dandelions Displace a Native Related Species Through Interspecific Pollen Transfer. Single-Pollen Genotyping (2011) pp. 83-99

3. Schuelke. An economic method for the fluorescent labeling of PCR fragments. Nature biotechnology (2000) vol. 18 (2) pp. 233-4

4. Vanschoenwinkel et al. Toward a global phylogeny of the "living fossil" crustacean order of the Notostraca. PLoS ONE (2012) vol. 7 (4) pp. e34998

5. Heffer and Pick. Rapid isolation of gene homologs across taxa: Efficient identification and isolation of gene orthologs from non-model organism genomes, a technical report. EvoDevo (2011) vol. 2 (1) pp. 7

On Nov 1, 2012, at 12:31 PM, Lauri Reinhold wrote:

> I was actually able to get 15 to go through and some that were over 30 mer. Good luck!
>
> On Thu, Nov 1, 2012 at 10:39 AM, Tristan Eversole <customerservice@trioptimum.com> wrote:
> So... wait, I can only order 10 oligos (5 primer sets)? Ooooh, tough choice.... :(
>
> On Oct 30, 2012, at 3:04 PM, Lauri Reinhold wrote:
>
> > Check this out!! They completely changed the promotion!!! And after talking to them, they refuse to honor the original promotion!
> >
> > On Thursday, October 25, 2012 9:56:16 PM UTC-4, Nathan McCorkle wrote:
> >
> >
> > ---------- Forwarded message ----------
> > From: Invitrogen <em...@invitrogenemail.com>
> > Date: Thu, Oct 25, 2012 at 1:31 PM
> > Subject: 7 days until Primer Day
> > To: nmz...@gmail.com
> >
> >
> >
> > 1 day only!
> >
> > On November 1, 2012 place an order for primers and you will only pay a penny for a primer!* Use the promotion code below when placing your order. This promotion is only valid for one day so mark your calendars now!
> >
> > Use promotion code: US – P915999 / CA – P915993
> >
> > Promotion excludes TaqMan® assays, TaqMan® primers, and TaqMan® probes.
> >
> > Get your free primers in 7 days
> >
> > FOR RESEARCH USE ONLY. NOT INTENDED FOR DIAGNOSTIC PROCEDURES.
> > © 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. In compliance with federal regulations, we hereby disclose that this email communication is for commercial purposes.
> >
> > *Terms and Conditions:
> > This promotion is open to customers in the US and Canada. Promotion excludes Taqman® assays, primers, and probes. Discount will apply to orders received by Life Technologies on November 1, 2012. Discount does not include shipping and handling. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply
> >
> > --
> > -Nathan
> >
> > --
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> >
> > <primer2.jpg>
>
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